Requirements for Laboratory Use of Chemicals cont'd

DOE-HDBK-1139/3-2005

Sources11

Consolidated Requirements12

4.3.6.2 Cylinders of gases that are greater than lecture

NFPA 45, 8.1.4.2

bottle size and have NFPA 45 Health Hazard

Ratings of 3 or 4, and cylinders of gases that have a

Health Hazard Rating of 2 without physiological

warning properties that are located in laboratory

units shall meet both the following conditions:

(1) Storage in approved continuously mechanically

ventilated gas cabinets; and

(2) Compliance with the requirements of Chapter

3, Toxic Gases, of NFPA 55.

4.3.6.3 Cylinders of pyrophoric gases that are greater than

NFPA 45, 8.1.4.3

lecture bottle size that are located in laboratory

units shall be kept in approved continuously

mechanically ventilated, sprinklered gas cabinets.

4.3.6.4 Regulator systems in laboratory operations shall be

NFPA 45, 8.1.5.2.1-2

equipped with two gauges, either on the regulator

or remote from the regulator, installed as to show

both the cylinder pressure and the outlet pressure.

When the source cylinder is outside of the

laboratory, a station regulator and gauge shall be

installed at the point of use to show the outlet

pressure.

4.3.6.5 Only in use cylinders shall be allowed in the

NFPA 45 8.1.6.3-4

immediate work area. A compressed gas cylinder

shall be considered in use when it is:

(1) connected through a regulator to deliver gas;

(2) connected to a manifold being used to deliver

gas; or

(3) a single cylinder secured as a reserve cylinder

alongside the cylinder described in (1).

4.3.6.6 The maximum internal volume (water volume) of

NFPA 45, 8.1.6.5

all cylinders in each of the listed classifications, in

use in the laboratory work area, shall comply with

the following [based on internal cylinder volume at

21, 101 kPa (70F, 1 atm)]:

(a) Maximum Quantity of Flammable Gases.

(1)

For a laboratory work areas of 500 ft or

less, the internal cylinder volume in

standard cubic feet equals 6.0.

(2)

For a laboratory work area greater than

500 ft or less, the internal cylinder

volume is 0.012 ft per ft lab work area.