Appendix A Listing of Equipment and Supplies

DOE-SPEC-1142-2001

APPENDIX A

LISTING OF EQUIPMENT AND SUPPLIES

Plastic disposable sterile culture tubes, 16 x 125 mm, with screw cap

Pipettes, serological with plug, plastic, sterile and individually wrapped (1, 5, and 10 ml)

Multi-channel micropipettors calibrated to deliver microliter quantities of medium and reagents

Microtest 111 tissue culture plate and lid, 96 well, 0.32 ml/well (round or flat bottom)

Note: Until such time as a definitive study showing a conclusive advantage to round vs. flat,

either is usable without prejudice.

CO2 incubator

Laminar flow hood

Sterile, cotton plugged Pasteur pipettes

Hemocytometer or Coulter Counter

Microscope

15 ml and 50 ml conical centrifuge tubes

96 well cell harvester

Gas ionization or liquid scintillation counter for detecting beta emissions from tritiated

thymidine.

Note: The items above are recommended for simplicity. Equivalents are acceptable. Each

laboratory conducts usual procedures to assure satisfactory operation of all equipment.

OTHER NOTES OF INTEREST

Material safety data sheets (MSDSs) are available for all chemicals and laboratory reagents and

should be read by all personnel before performing this assay.