Other Notes of Interest

DOE-SPEC-1142-2001

RPMI-1640, L-glutamine, penicillin-streptomycin, sterile phosphate buffered saline solution, and

Ficol-Hypaque are sterile tissue culture reagents. When used with appropriate tissue culture

laboratory practices, they pose no known safety, health, or disposal hazards.

Beta Plate Stint or its equivalent is a biodegradable scintillation fluid. It is stored and used in a

chemical fume hood with appropriate protective clothing, gloves, and face shield. If a direct

counting method is employed, this does not apply.

Human serum, type AB has been tested by an FDA approved method and found non-reactive for

the presence of HBsAG and antibody to HIV by the supplier. However, it is derived from human

source material and will be handled observing the same safety precautions used when handling

any potentially infectious material.

Tritiated thymidine emits low energy beta radiation and shall be used with appropriate exposure

controls and personal protective equipment. Policies for the use and disposal of radioactive

reagents and laboratory ware are found in the guidelines prepared by the institution's safety and

health protection offices and shall comply with accepted practices.

Beryllium sulfate is an extremely hazardous chemical. It is, based upon animal data, assumed to

be a potential human carcinogen. It is a strong irritant; contact with skin, eyes, and mucous

membranes must be avoided. Consult MSDS before handling this chemical.

Computer software written in language Splus is available for performing the calculations

described in this specification (see appendix of ORNL-6818 and

straightforward so that simple programming can be used to implement it in any spreadsheet.

Two Excel programs that use the data in Table B-I to illustrate the calculations are available on

page 12 of the Tritiated Thymidine Beryllium Lymphocyte Proliferation Test Electronic