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DOE-SPEC-1142-2001
CONTENTS
PARAGRAPH
PAGE
FOREWORD..........................................................................................v
1.
SCOPE ........................................................................................................................ 1
2.
BACKGROUND ........................................................................................................ 1
3.
PRINCIPLE ................................................................................................................ 1
4.
APPLICATION ......................................................................................................... 2
5.
TRITIATED THYMIDINE PROCEDURE FOR DETERMINING
BERYLLIUM SENSITIZATION .............................................................................. 2
5.1
REAGENTS AND EQUIPMENT ................................................................. 2
5.2
PREPARATION OF REAGENTS ................................................................ 3
5.3
QUALITY CONTROL .................................................................................. 3
6.  TRITIATED THYMIDINE BeLPT: BLOOD SPECIMEN....................................... 4
6.1
SAMPLE COLLECTION PROCEDURE ..................................................... 4
6.2
SAMPLE PREPARATION............................................................................ 5
7.  TRITIATED THYMIDINE BeLPT: LAVAGE SPECIMEN .................................... 6
7.1
SAMPLE COLLECTION .............................................................................. 6
7.2
SAMPLE PREPARTION .............................................................................. 6
7.3
SAMPLE FORM............................................................................................ 6
7.4
ANALYTICAL PROCEDURE ..................................................................... 7
8.  CALCULATIONS...................................................................................................... 8
9.  RESULTS ................................................................................................................... 8
10. REFERENCES ......................................................................................................... 10
LIST OF APPENDEXES
APPENDIX A
LISTING OF EQUIPMENT AND SUPPLIES ............................ 11
APPENDIX B
CALCULATIONS FOR THE BeLPT .......................................... 13
APPENDIX C
SAMPLE REPORTS..................................................................... 21
iii

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